00 ............... ........ ? 

SENATE......................................... 

Subject... 

NEW

. .

.COURSE.- PROPOSAL .- 

?

BICH 412-3 

.

SENATE COMMITTEE ... ON. 

. UNDERGRADUATE 

STUDIES. . 

Date ... ..NOVEMBER 

. 13,. .1975 

MOTION: ? 

"That Senate approve and recommend approval to the 

Board of Governors, as stated in S.75-181, BICH 412-3 - 

Enzymology." 

(Note: If BICH 412-3 is introduced, BICH 411-2 will 

be discontinued.) 

Action taken by the Senate Committee on Undergraduate 

Studies at its meeting of November 12th, 1975 gives rise to the 

following motion: 

MOTION 

That Senate approve and recommend approval to the 

Board of Governors BICH. 412-3 - Enzymology. 

Daniel R. Birch 

ams

att. 

..

* At its meeting of October 28, 1975, the Faculty of Science approved the 

proposal for a new course BICH 412-3, Enzymology. This course represents 

the addition of one weekly lecture to the 

a

lready-existing lab course, 

BICH 411-2, and will replace that course. 

The supporting documentation is forwarded herewith for consideration by 

SCuS. 

/pel 

o

* If the above proposal is approved, it will have the effect of changing 

the calendar entry for 

401-3 as follows: 

Delete: 

411-2 will ordinarily be taken 

c

oncurrently, but may 

be taken subsequent to 

401-3. 

Add: ? 

412-3 will ordinarily be taken concurrently, but may 

be taken subsequent to 

401-3. 

SENATE COMMITTEE ON UNDERGRADUATE 

STUDIES      

- ?

-

NEW COURSE PROPOSAL FORM 

Program 

1.. Calendar 

Information                  

wt

:Biochemistry 

Abbreviation Code:BICH 

?

Course Number: ? 

Credit Hours: 

?

Vector:1-O-'4- 

Title of 

Course: ? 

Enzyiiiology 

Calendar 

Description 

of Course: ? 

&zyme isolation 

and assay 

procedures; 

energy 

of activation; enzyme kinetics and inhibition; 

mechanisms of 

eflz3rmiC 

reactions; allosteric enzymes. 

Nature of Course 

?

Lecture/Laboratory 

Prerequisites (or special instructions): 

BICH 301- (or ISC 301-3) and BICH 

(or BICH 

312-2) 

sr

?

,1%

?

(3/c-N 

4'//-Z ? 

y S

?

What course (courses), if any, is being dropped from the calendar if this course is 

approved: ? 

BICH 1+11-2 

2.

Scheduling 

How frequently will the course be offered? 

once a year 

Semester in which the course will first be offered? 

Which of your present faculty would be available to make the proposed offering 

• ? 

possible? 

1

-1.1- 

1

Richards, 

S. Aronoff, J.S. Barlow, R.J. Cushley 

3.

Objectives of the Course 

?

The addition of one lecture 

p er week to the 

currently existing enzymology laboratory course (BICI-t 

1 +11-2) is the 

best way to correlate lecture material with laboratory work. The 

lecture material does not fit conveniently into any of the existing 

:i ecture courses. Attem 

p

ts to do so have led to serious scheduling 

p

roblems with the corres 

p

onding laboratory course. 

4.

Budgetary and Space Requirements (for information only) 

What additional resources will be required in the following areas: 

Faculty 

.

Nil

Staff Nil 

Library Nil 

Audio Visual Nil 

Space 

Lecture (11wk); laboratory soace is available in C9011+ 

Equipment 

(See attached sheet on Laboratory Experiments) 

5.

Approval 

Date:____________ 

?

Dean ? 

Chairman, SCUS 

Committee Chairman 

SCUS 73-34b:-- (When completing this form, for instructions see Memorandum SCUS 73-34a. 

Attach course outline). 

(1-0-4) 

Lecture Tonic 

1 ?

:action rate theOr\ 

r

; enerv of nctivation 

zyr:e 

?

ntirntion; 

?

of entrory effe-'t 

end

Concentaatjon 

auantity: 

).

nrocndre 

o

p

timum 

?

cnciitions 

n:othods 

of

of detoruj.njn 

assay (aI, 

?

?

temperture, 

enze cuality 

and

L

Revicw 

com

p

lex

of

formation. 

chemical kinetics; the 

steady-state anPro 

?

mtiôn and 

Enzyme kinetics; 

?

JchaeUs-• . 

:enton 

and Linewe aver -'ri( treatments 

6

Enzyme inhibition (I) 

Enzyme inhibition (II) 

8

Past reaction kinetics 

Studies on monosubstrate reaction 

mechanisms 

10

Studies on hisub 

g

trate reaction mechanisms 

11

Sigmoidal knet1c 

12

Ailosteric enzymes (I) 

13

Allosteric enzymes (II) 

BICH 412-3, Enzymology 

Laboratory Experiments 

It is anticipated that quite a bit of freedom will be allowed for the 

students in choosing laboratory experiments, as is currently the case for 

which 

BICH 411-2. 

he or she 

The

wishes 

student 

to

will 

isolate 

choose 

and

an

purify. 

enzyme, 

He/she 

from 

will 

a list 

then 

of

carry 

pos

sibilities, 

out

enzyme assays and other enzymic studies on his/her isolated enzyme. (If 

no activity is obtained, he/she may be given a 

c

ommercially obtained 

enzyme for his/her enzymic studies.) These studies could include: 

1.

Energy of 

a

ctivation, devaluation and pH optimum. 

2.

Enzyme kinetics: 

Michaelis-Menton 

and Lineweaver_Burk treatments. 

3.

. Enzyme inhibition. 

?

.

The

experiments 

should take about 2/3 of the semester. For the 

r

emaining 1/3, experiments in advanced areas ofenz 

Ai ? 

ymology are planned.                                        

•

?

gan, the student would have fredclom in selecting the experiments, which 

would include: 

I.

?

Bisubstrate reaction mechanisms 

• ? 2. ? 

Allosteric enzymes 

3.

?

Fast reaction kinetics 

The latter experiment would require a stopped-flow or relaxation 

but

kinetics 

funds 

a

will 

pparatus. 

be sought 

Such 

in

an

the

apparatus 

budget 

will 

for Fall 

not be 

1977. 

available 

A good portion 

for Fall 

of

1976

the apparatus can be built in the SFU workshop. 

.